Advancements in Crop Yield Improvement through Genetic Engineering

Introduction

Agriculture has been the backbone of human civilization; it provides food, raw materials, and livelihood for millions. However, traditional agricultural practices of breeding stock over thousands of years have now reached their biological and practical limits in crop yield improvement. With the increasing effects of climate change, dwindling arable land resources, and the imperative for sustainable food production, genetic engineering has emerged as a dominant tool in mitigating these effects. This review highlights recent advances in crop yield improvement through genetic engineering and evaluates the possibility of this technology revolutionizing contemporary agriculture.¹

It is quite challenging to feed a rising population over time to nearly 10 billion by 2050 and hence critical to dramatically increase agricultural productivity. Two fundamental approaches have been used as the main route to raise crop yields for the past century: selective breeding and hybridization 2. Nevertheless, the two methods usually take long and are limited by the natural limits of plant genetics. It’s in the face of environmental stressors like global change, dwindling arable land, and more frequent pests and diseases that the call for more innovative means of farming is continually growing towards making sustainable food production imperative.³

Genetic engineering has quickly become one of the most high-priority technologies in modern agriculture, promising unprecedented precision and efficiency to crop improvement. Genetic engineering allows for the direct modification of a plant’s genetic makeup, introducing desirable traits that significantly improve crop yield, disease resistance, and environmental adaptability. CRISPR-Cas9, transgenic methods, and RNA interference (RNAi) are some of the technologies that have revolutionized scientists’ approaches to crop development by allowing rapid, targeted alterations to the plant genome.²

Figure 1: Advancement in Crop Yield improvement through genetic algorithm Click here to view Figure

This article discusses the key advancements in genetic engineering that find direct ways to crop yield improvement. It considers the potential of different GE technologies, their applications in staple crops, and environmental, ethical, and regulatory considerations related to the use of GE technologies. Challenges and future directions in applying genetic engineering to solve the increased demand for more food produced while promoting sustainability are also discussed.

Genetic Engineering Technologies for Crop Yield Enhancement

CRISPR-Cas9 Technology

The CRISPR-Cas9 is a novel genome-editing technique that has transformed the era of precise plant DNA modification. Targeting a specific gene that can influence crop growth, resistance to pests, or adaptability to certain environmental conditions is characteristic of this technology. One of the most highly talked-about, rapid genetic engineering technologies that have revolutionized crop improvement owing to their remarkable precision, efficiency, and versatility is CRISPR-Cas9. As a natural mechanism that bacteria have evolved in order to defend themselves, Clustered Regularly Interspaced Short Palindromic Repeats, or CRISPR, now work in concert with the enzyme Cas9, allowing scientists to edit specific pieces of DNA in plants and other organisms ⁴. This capability has proved invaluable in increasing crop yield production, strengthening resistance against pests and diseases, and enhancing crops’ resistance to environmental stressors. Thus, CRISPR-Cas9 opens new doors for the future of sustainable agriculture through the possibility of fine changes to a plant’s genetic makeup.⁵

Essentially, the CRISPR-Cas9 system works by having a guide RNA target the Cas9 enzyme to a specific point in the plant’s genome where it cuts the DNA. From there, DNA repair mechanisms in the plant can take over, and scientists can add desirable traits or remove unwanted genes. The level of precision is further advanced compared to conventional methods, which rely on crossing at a more general genetic level and very often take a number of generations for trial and error. With CRISPR-Cas9, modifications of specific interest can be made in one generation; hence, traits can be developed much faster in crops ⁶. Figure 2 shows application of CRISPR-Cas9 technology.

Figure 2: Application of CRISPR-Cas9 Technology Click here to view Figure

Table 1: Applications of CRISPR-Cas9 technology in Crop Improvement

Application	Description	Example Crops	Key Benefits
Disease Resistance	Editing genes to make crops resistant to specific plant diseases.	Rice, Wheat, Tomato	Reduced crop loss, improved yield
Insect Pest Resistance	Targeting genes that control plant susceptibility to insect pests.	Cotton, Maize, Soybean	Reduced dependency on chemical pesticides
Drought Tolerance	Modifying genes related to water retention, root development, and stress response.	Rice, Sorghum, Maize	Enhanced survival under drought conditions
Nutrient Enhancement	Increasing the content of essential nutrients, such as vitamins or minerals, in crops.	Rice, Wheat, Potato	Improved nutritional value for human consumption
Herbicide Resistance	Modifying crops to tolerate specific herbicides without damaging the crop itself.	Soybean, Corn, Canola	Better weed control without harming crops
Improvement in Photosynthesis	Enhancing the efficiency of photosynthesis to improve crop growth and yield.	Rice, Wheat, Potato	Increased yield under optimal conditions
Quality Traits (e.g., taste, color)	Modifying genes related to the sensory attributes of crops, such as flavor, color, and texture.	Tomato, Grapes, Banana	Enhanced marketability and consumer preference
Abiotic Stress Tolerance (salinity)	Editing genes to improve crop tolerance to salt stress, making them more adaptable to saline environments.	Rice, Wheat, Barley	Increased productivity in saline soils
Seedless Fruit Development	Editing genes to create seedless varieties of fruit crops.	Grapes, Watermelon, Citrus	Improved consumer experience and yield
Fast-tracking Breeding	Using CRISPR to accelerate the breeding process by editing specific genes of interest.	Multiple crops (e.g., Maize, Wheat, Rice)	Faster development of improved crop varieties

Perhaps one of the greatest applications of CRISPR-Cas9 in agriculture has been its impact on the improvement of photosynthetic efficiency and directly on crop biomass and yield as shown in Table 1 with detailed application⁷. This is realized through gene modification of the genes that take part in photosynthesis. Such plants can henceforth harvest sunlight as energy for better productivity. Studies have already shown that rice varieties edited using CRISPR have improved photosynthesis and hence higher grain yield. An important hope for resolving food security problems will thus be assured as the world population continues to grow .⁸

Besides improving photosynthesis, CRISPR-Cas9 has played a significant role in producing drought-resistant heat-tolerant crops, which can also survive exceedingly well in deficient soils. Scientists have achieved genetic modification of genes in charge of a plant’s response to stress that culminated in drought-resistant maize and other crops sustaining higher yields even during adverse environmental conditions. The technology also incorporates enhancements in nutrient use efficiency, so that the plants will utilize key nutrients like nitrogen far more economically. In this manner crop yield improves while the amount of chemical fertilizers used diminishes, making farming styles far more sustainable.⁹

Another critically important advantage with CRISPR-Cas9 relates to improved resistance to pests and diseases. For instance, tomatoes, wheat, and bananas have been edited with the CRISPR tool in order to make them resistant to diseases caused by bacterial and fungal infections. This reduces reliance on chemical pesticides, lowers the risk of loss, and makes agriculture even greener. The changes that this ensures occur without affecting any other valuable characteristic in the plant.¹⁰

Despite all the advantages CRISPR-Cas9 has, there have been several challenges it has faced. One of them is an off-target effect about modifying the genome in places it was not intended to and that can cause some unforeseen problems in crop performance or crop safety. Although ongoing efforts try as much as possible to reduce these risks, this is still a technical hurdle that needs to be overcome. Additionally, there isn’t yet a uniform regulatory approach toward gene-edited crops among countries. While some parts of the world adopt CRISPR-edited crops as a nontransgenic, others create regulatory barriers that limit its spread worldwide. Public perception also plays a role: Skepticism among consumers concerning GMOs often extends to CRISPR-edited crops, mainly because the technique is still more precise than genetic engineering.^4,10

Despite all the advantages CRISPR-Cas9 has, there have been several challenges it has faced. One of them is an off-target effect about modifying the genome in places it was not intended to and that can cause some unforeseen problems in crop performance or crop safety. Although ongoing efforts try as much as possible to reduce these risks, this is still a technical hurdle that needs to be overcome. Additionally, there isn’t yet a uniform regulatory approach toward gene-edited crops among countries. While some parts of the world adopt CRISPR-edited crops as a nontransgenic, others create regulatory barriers that limit its spread worldwide. Public perception also plays a role: Skepticism among consumers concerning GMOs often extends to CRISPR-edited crops, mainly because the technique is still more precise than genetic engineering.¹¹

Transgenic Crop

Genetically modified crops involve the introduction of foreign genes into plant genomes to express specific traits that might improve yield. These traits may include resistance against pests and diseases, better use of nutrients, or tolerance to extreme environmental conditions. Transgenic crops include Bt corn, which produces a bacterial toxin toxic to certain insects and have been widely embraced to protect crops against more pests than other crops as well as for increased yields. Transgenic crops are one of the genetically modified organisms whereby genes from a selected organism are transferred into the genome of another organism, generally to enhance desirable characteristics such as yield, resistance towards pests, or tolerance of environmental stresses. Unlike the conventional breeding methods, whereby related species are crossed to produce offspring, transgenic technology allows scientists to bypass natural barriers to reproduction and, directly transfer genes from one unrelated species to another with the creation of plants bearing new characteristics that could not have been obtained through conventional breeding.¹²

Figure 3: Applications of Transgenic Technology Click here to view Figure

Modern Agriculture Transgenic crops have revolutionized modern agriculture, providing solutions to some of the most daunting challenges facing the farmer. Applications of Transgenic Technology is shown in Figure 3.Transgenic crops can significantly reduce the usage of chemical pesticides and herbicides since they contain genes conferring resistance to specific pests or diseases, besides herbicides. T cotton is one such example-this contains a gene from the bacterium Bacillus thuringiensis which produces proteins toxic to certain types of pests. It reduces reliance on chemical insecticides, hence costs in terms of produce and environmental pollution through less usage of chemicals.¹³

Transgenic crops were also engineered to enhance tolerance against environmental stresses like drought, salinity, and high temperatures, which are increasingly becoming prevalent these days because of climate change. These examples include drought-tolerant maize and salt-resistant rice; under stressful growing conditions, these crops ensure that they yield at high levels, thus becoming a critical instrument for ensuring food security, especially in areas with incidences of water scarcity and soil degradation. Transgenic crops that can tolerate bad conditions bring agricultural productivity to areas that could not previously sustain farming.¹²

Besides strengthened resistance to pests and stresses from the environment, the transgenic crops are designed to enhance nutritional content. For instance, “Golden Rice” is a genetically engineered variety of rice that can produce beta-carotene, a precursor to vitamin A. This crop has been developed in order to contribute to the eradication of vitamin A deficiency: this is a huge issue in the health of millions of people all over developing regions. It is indeed a splendid example of how genetic engineering can be mobilized for fighting malnutrition and improving human health through agriculture.¹⁴

This notwithstanding, success and merits aside, transgenic crops remain widely debated and questioned. Some of the critical concerns relate to the environmental impact: unintended ecological consequence of gene flow between the transgenic crop and the wild relatives, and the generation of resistance in pests and weeds. For example, the intensive deployment of Bt crops has led to the emergence of resistant populations of insects in certain areas. Such instances raise crucial questions about the more long-term sustainability of transgenic pest control practices.¹²

Regulatory and public acceptance issues are also at the forefront of transgenic crop adoption. There are country-specific policies on GMOs, allowing some places to open their arms to embrace this technology, while others have the control mechanisms or will not allow GMOs and prohibit them either in cultivation or use. Mostly, opinions of the public on transgenic crops relate to food safety and associated environmental risks and ethics, whose concerns have mounted regulatory barriers against GMO crops, especially in regions like Europe.¹⁵

Transgenic crops are the best technological mileage for agriculture to provide solutions that offer high crop yield, pest resistance, environmental resilience, and nutritional content. However, environmental impact, resistance management, and public acceptance are part of its challenge. Continuous research and responsible implementation of transgenic crops can be a step forward in addressing some of these concerns while maximizing its benefit to global food security and sustainable farming practices.

RNA Interference (RNAi)

RNA interference, or RNAi, is the silencing method of specific genes that negatively influence plant growth or resistance. It does this by targeting and “turning off” these genes. RNAi is a promising tool to upgrade crop resilience to diseases, thus improving the yields by curtailing losses.¹⁶

RNAi is an RNA-based technology in genetic engineering and crop improvement that controls gene expression by silencing or inhibiting specific genes. Discovery of RNAi occurred late in the last century. It revolutionized the methods used to protect and improve crops, as scientists are now able to target and silence unwanted genes responsible for unwanted traits, such as susceptibility to pests or diseases. It interferes with the translation process of messenger RNA (mRNA) by small RNA molecules and completely blocks the production of specific proteins that control various plant functions.¹⁷

There are two main types of RNAs that silence genes: siRNA which induces interference in the target mRNA due to a complex called RNA-induced silencing complex, and miRNA, which guides the RISC to the target mRNA strand where it binds and prevents the translation of the mRNA into proteins. This capability in plants can be exploited in manipulating down the expression of genes that render crops susceptible to pests, diseases, or environmental stresses, thereby increasing crop resistance and yield.¹⁸

One of the most important applications of RNAi in agriculture is pest control. This prevents chemical pesticides being applied directly, and instead, RNAi can target pesticide application more sustainably with fewer environmental impacts. By introducing silencing genes necessary for survival or reproductive purposes for these pests, crops can be protected from them without having any negative effects on other beneficial insects as well as the environment. An example is the use of RNAi to create genetically engineered corn for silencing specific genes in the western corn rootworm, a major pest responsible for most of the yield loss. This kind of pest control diminishes chemical use in insecticides and moves agriculture forward towards more sustainable agriculture.¹⁹ Figure 4 highlights applications of RNA Interference (RNAi).

Figure 4: Applications of RNA Interference (RNAi) Click here to view Figure

RNAi has further been utilized to enhance resistance to diseases in crops. Most viruses, bacteria, and fungi, as well as most plant pathogens, have genes specific to infecting or destroying plants. Scientists use RNAi to knock out pathogen-related genes, making crops not susceptible to infection. For example, using RNAi, researchers were able to produce papaya varieties resistant to the PRSV virus that infects papayas. These transgenic papayas are resistant to the disease due to the silencing of viral genes responsible for infection, thus saving the farmer from losses due to crop failure.²⁰

Apart from resistance to pests and diseases, RNAi has been used in crops for enhancing some of the desirable traits such as nutrient content, shelf life, and tolerance to stresses. For instance, RNAi can be used to reduce the levels of enzymes that make fruits like tomatoes turn soft and rot faster, hence remain fresh for a longer time. RNAi can also be used to suppress genes responsible for making crops extremely susceptible to either drought or excessive heat, thereby enhancing stress tolerance and stability of yields even under the most adverse growing conditions.

The defining characteristic of RNAi technology is its precision. This method is dissimilar to the usual methods of genetic modification, since it does not involve a foreign DNA source; instead, it results from gene silencing through the RNAi mechanism. Therefore, RNAi can be considered a more natural means toward genetic improvement, since it manipulates only certain endogenous genes, minimizing risks of unintended consequences and off-target effects, thus enabling scientists to achieve the desirable plant characteristic without compromising other important plant characteristics. For instance, even though it does not change the DNA of the plant in reality, RNAi permanently does not change the DNA of the plant since silencing often lasts short and is decided on specific needs RNAi acts.

Although much promise is still held by RNAi technology, however, it is not free from issues. The off-target effects characterize it whereby unintended genes are silenced thereby often causing unwanted changes in the phenotype of a plant. The effectiveness of RNAi also differs since some species of the organism or pest targeted may have mechanisms that allow them to evade RNAi-mediated silencing. There are also concerns with the stability and long-term effects of RNAi-based modification in the field, much like regulatory and public acceptance issues faced by other GMOs.

Therefore, RNAi is the latest crop improvement technology that has entered this world and given the scientific fraternity an accurate, sustainable, and environmentally friendly way of generating resistance against pests and diseases, crop traits, and eventually agricultural productivity. Though there are challenges in the use of RNAi, it still has tremendous scope to help meet the increasing needs of food security and sustainable farming in this finite world which suffers from global climate change and resource constraints. It is expected that RNAi technology will advance with new research and developments, and it will likely be part of agriculture in the future.²¹

Applications of Genetic Engineering in Important Crops

Rice (Oryza sativa)

Rice has been an object of long-standing interest due to its role as the staple food crop for more than half the world’s population, and genetic engineering efforts have targeted it as the first primary crop target. CRISPR and transgenic approaches were utilized to introduce improved nitrogen use efficiency, enhanced photosynthetic efficiency, and drought-stress resistance into rice to increase yield. Genetic engineering has proved to be a revolutionary factor in rice (Oryza sativa), one of the world’s most important staple crops. Therefore, with an increasing global demand for food and new situations brought about by climate change, genetic engineering offers strong tools for improving the productivity of rice, nutritional quality, and environmental resistances. Genetic engineering in rice can be very useful for introducing traits of nutrient deficiency, pest infestation, disease resistance, or water scarcity²². Some of the key applications of genetic engineering in rice production include the following:

Nutritional Enhancement

This is one of the largest contributions of genetic engineering to rice-from nutrification of varieties of rice. For instance, Golden Rice is genetically altered to have high levels of beta-carotene, the precursor to vitamin A. Vitamin A deficiency is a major public health issue in many developing countries, leading to preventable blindness and immunological deficiencies, especially among children. Golden Rice would therefore solve this problem by providing a biofortified source of vitamin A to reduce malnutrition and bring better public health outcomes in regions where rice is a dietary staple.The next one is genetic modification of rice for the production of enhanced iron and zinc content. This is because of a widespread problem of micronutrient deficiency in the populations that consume rice for a significant amount of their diet. These innovations are going to play a very important role in combating malnutrition at a global level by improving the nutritional profile of rice through biofortification.²³

Pest Resistance

Such insect pests as the rice stem borer can reduce yields from rice significantly. This necessitates much higher production costs and much higher usage of pesticides. Genetic engineering has now enabled the development of pest-resistant varieties of rice that require much less chemical use in pesticide application. For example, Bt rice with a gene from the bacterium Bacillus thuringiensis, now secretes toxic protein to certain pests. It is harmless to humans and these beneficial insects. Bt rice has demonstrated to provide excellent protection against stem borers and other pests through its enhanced yield combined with reducing pesticide use on the environment.²⁴

Disease Resistance

Other diseases, including bacterial blight, rice blast, and sheath blight, can devastate and become yield-reducing for rice. Through genetic engineering, genes that confer immunity or tolerance to these diseases have been introduced into the rice, thus making the varieties more resistant. Actually, resistance to bacterial blight in rice has been engineered by transferring such resistance genes from wild rice or other related species into the crop. Even these genetically modified rice plants would not lead to outbreaks of disease, thereby facilitating stable yields and deterring the wanton application of chemical treatments.²⁵

Drought and Flood Tolerance

Generally, extreme weather conditions may result in drought or floods, which are some of the significant problems affecting crop production, but genetic modification provides measures for remedial action by introducing drought and flood tolerance into the genes of rice crops. For instance, drought-resistant ones are engineered to carry genes that will help enhance water-use efficiency in a way that the plant lives and is productive under water-limited environments. ²⁶

Similar gains have been made with rice varieties that are tolerant of submergence. This was made possible by introducing the Sub1A gene into the plant to survive the period of prolonged flooding. The variety will be beneficial to areas prone to flash flooding, as yields that have completely been lost by the traditional varieties would otherwise have been produced. All these stress-tolerant improvements in rice can actually allow farmers to adjust to such scenarios due to climate variability, thus ensuring proper rice production in sensitive areas more reliably.

Herbicide Tolerance

Weeds compete with the rice plants for nutrients, water and light; herbicide tolerant varieties of rice can be engineered for tolerance to broad-spectrum herbicides and sprayed with them for killing weeds and do not harm the crop of rice. This simplifies weed management in rice and reduces labor and chemical inputs. For example, herbicide-tolerant rice engineered with the glyphosate or glufosinate herbicide system can help farmers weed more effectively far down in the canopy, and over time that can result in higher yields and less manual weeding labor.²⁷

Increasing Efficiency of Photosynthesis

Another big area where genetic engineering is being applied in rice is in increasing the efficiency of photosynthesis. Scientists have been engineering the expression of some genes involved in the pathway of photosynthesis to enhance the speed with which a rice plant can begin to produce energy from sunlight, thereby increasing the yield through more biomass. Engineers are working on the molecular replacement of the more efficient version of the enzyme RuBisCO, which is crucial in the carbon fixation of the process. This innovation has the potential to boost rice productivity dramatically, thereby being one of the possible remedies for the growing food demand due to the increasing population of the world.²⁸

Salinity Tolerance

Soil salinity is among the biggest problems in many regions with paddy cultivation. This is the case with coastal areas and regions affected by salinity introduced through irrigation practices. Soil salinity above certain threshold levels inhibits rice plant growth and affects production. With genetic engineering, varieties of rice more tolerant to field saline conditions have been developed by the introduction of genes that aid the plant in maintaining the ion balance and osmotic pressure. The salt-tolerant varieties of rice will be able to farm rice on salinized areas, thereby expanding the possibility of rice cultivation in marginal lands.²⁹

Hybrid Rice Production

Although hybrid rice has greatly contributed to an increasing yield in several regions around the world, the production of hybrid seeds is highly laborious and expensive. Genetic engineering was used to make hybrid rice production much easier by developing genes that would control the fertility or sterility of the rice plant. It has been developed, for instance, ‘hybrid’ varieties that can shift from male fertility to sterility under environmental conditions or chemical treatments, making the hybrid seed production easier and less costly. This can significantly increase yields of rice, which is the staple crop in parts of the world, thus contributing to food security.³⁰

Maize (Zea mays)

Maize is another crop that also yielded great benefits through genetic engineering, particularly with Bt maize and those possessing drought tolerance. Genetic modification has improved the more environmental stress tolerance capability of maize while sustaining high productivity levels. Genetic engineering has significantly advanced the development of maize (Zea mays), one of the world’s most important staple crops, providing solutions to challenges related to crop productivity, pest resistance, climate resilience, and nutritional enhancement. By introducing specific traits through genetic modifications, scientists have improved maize’s performance in various agricultural contexts, making it more efficient and sustainable to cultivate.³¹ Below are some key applications of genetic engineering in maize cultivation

Insect Resistance

One of the earliest and most impactful applications of genetic engineering in maize is the development of insect-resistant varieties. The most notable example is Bt maize, which contains a gene from the bacterium Bacillus thuringiensis (Bt). This gene enables maize to produce a protein that is toxic to specific insect pests, such as the European corn borer and corn rootworm, but is harmless to humans and non-target organisms. Bt maize has significantly reduced crop losses due to insect damage, increased yields, and decreased the reliance on chemical insecticides, promoting more sustainable pest management practices.³²

Herbicide Tolerance

Weed control is a major challenge in maize farming, and herbicide-resistant genetically engineered maize varieties have proven effective in addressing this issue. Herbicide-tolerant maize, such as those resistant to glyphosate or glufosinate, allows farmers to apply broad-spectrum herbicides that kill weeds without damaging the maize crop. This simplifies weed management, reduces the need for multiple herbicide applications, and lowers production costs. Glyphosate-tolerant maize (commonly known as Roundup Ready maize) has been widely adopted due to its effectiveness in controlling a broad range of weeds, thereby improving crop yield and reducing labor-intensive weeding practices.³³

Drought Tolerance

With the growing impact of climate change, developing crops that can withstand drought conditions has become a priority for agricultural sustainability. Genetic engineering has been used to create drought-tolerant maize varieties, such as the DroughtGard maize developed by Monsanto. These maize varieties contain genes that enhance the plant’s ability to maintain productivity and survive under water-limited conditions. Drought-tolerant maize has been particularly beneficial in regions prone to dry conditions, such as parts of Sub-Saharan Africa and the U.S. Midwest, where it helps stabilize maize production even in drought-prone growing seasons.³⁴

Nutritional Enhancement

Biofortification through genetic engineering has allowed the development of nutritionally enhanced maize varieties, addressing micronutrient deficiencies in regions where maize is a dietary staple. One example is maize engineered to increase the levels of essential amino acids, such as lysine, which are naturally limited in conventional maize varieties. These nutritionally enhanced maize strains, known as Quality Protein Maize (QPM), have the potential to improve the nutritional intake of populations that rely heavily on maize for sustenance. Furthermore, genetic engineering has been used to develop maize varieties with higher levels of vitamins and minerals. Efforts are underway to create maize that contains higher levels of provitamin A (beta-carotene) to address vitamin A deficiency, a common issue in many developing countries. Similar to the Golden Rice initiative, biofortified maize could help combat malnutrition and improve public health outcomes.²³

Disease Resistance

Maize is susceptible to various diseases caused by viruses, fungi, and bacteria, which can lead to significant yield losses. Genetic engineering has enabled the development of maize varieties that are resistant to major diseases, reducing the need for chemical treatments and ensuring more reliable crop production. For example, maize varieties resistant to fungal pathogens that cause diseases like maize streak virus (MSV) and gray leaf spot (GLS) have been developed by introducing genes that enhance the plant’s immune response. Such disease-resistant varieties help reduce the impact of crop diseases, ensuring more stable yields and lowering production costs for farmers.²⁵

Improved Yield and Photosynthesis Efficiency

Another promising area of genetic engineering in maize is the improvement of photosynthesis efficiency to enhance biomass production and yield. By modifying genes involved in the photosynthetic process, scientists aim to make maize plants more efficient at converting sunlight into energy. This can lead to increased grain production without the need for additional inputs such as fertilizers or water. Genetic engineering efforts are also focused on optimizing the carbon fixation pathway in maize, which could further boost photosynthetic efficiency and contribute to higher yields in diverse environmental conditions.²⁸

Biofuel Production

Maize is a key crop used in the production of biofuels, particularly ethanol. Genetic engineering has played a role in optimizing maize for biofuel production by enhancing its starch content and modifying the plant’s metabolic pathways to increase the efficiency of converting biomass into ethanol. Genetically modified maize varieties with higher fermentable starch content can be processed more efficiently, making biofuel production more cost-effective and sustainable. Additionally, research is ongoing to develop maize varieties that produce cellulases and other enzymes directly in the plant, which could simplify the process of converting plant material into biofuels.³⁵

Cold and Heat Tolerance

As global temperatures fluctuate, maize crops in many regions are increasingly exposed to temperature extremes that can affect growth and yield. Genetic engineering has been used to develop maize varieties that are more tolerant to both cold and heat stress. Cold-tolerant maize varieties allow for earlier planting in regions with colder climates, extending the growing season and improving overall productivity. Conversely, heat-tolerant maize varieties are engineered to maintain growth and reproductive development during periods of high temperatures, reducing the risk of yield losses due to heat waves.³⁶

Nitrogen Use Efficiency

Maize requires significant amounts of nitrogen for optimal growth, and conventional farming practices often rely on synthetic nitrogen fertilizers, which can lead to environmental issues such as water pollution and greenhouse gas emissions. Genetic engineering has been employed to develop maize varieties with improved nitrogen use efficiency (NUE), allowing the plants to utilize available nitrogen more effectively and reducing the need for synthetic fertilizers. These genetically engineered maize varieties help lower production costs for farmers and minimize the environmental impact of nitrogen fertilization, contributing to more sustainable agricultural practices.³⁷

Hybrid Seed Production

Genetic engineering has also streamlined the production of hybrid maize seeds, which are known for their higher yields and vigor compared to open-pollinated varieties. By manipulating genes involved in fertility and sterility, scientists have developed maize varieties that make the production of hybrid seeds more efficient. This reduces the cost and labor associated with producing hybrid seeds, making them more accessible to farmers. Hybrid maize has played a major role in increasing global maize production, and genetic engineering continues to improve the efficiency of hybrid seed production systems.³⁸

Wheat (Triticum aestivum)

The latest research with wheat genome editing using CRISPR have produced even more improved varieties toward greater resistance against fungal diseases such as rust, which could sometimes be very destructive on yields. There is also some promise about boosting wheat’s tolerance to extreme climate conditions toward increasing crop stability and eventually yield.

The selection of major crops depends on agronomic, ecological, economic, and social factors to ensure suitability for specific regions and maximize productivity.

Agronomic Factors

Key considerations include high yield potential, appropriate growth duration, resistance to pests, diseases, and abiotic stresses like drought and salinity. The crop must match the local soil type and water availability.

Ecological Factors

Crops must suit the region’s climate, including temperature, rainfall, and humidity, while minimizing environmental impact and adapting to climate change.

Economic Factors

Market demand, profitability, low input costs, export potential, and value addition through processing drive crop selection. Crops must provide good financial returns to farmers.

Social and Cultural Factors

Crops should contribute to food security, align with cultural preferences, generate employment, and provide nutritional benefits. Support from government policies, such as subsidies and procurement programs, also influences choices.

Technological Factors

The availability of improved varieties, ease of mechanization, and access to research and extension services are critical for ensuring successful cultivation.

Environmental and Ethical Considerations

This is one of the key areas where the impact of genetic engineering in wheat (Triticum aestivum) significantly plays a role in agricultural productivity and food security. But along with these, there are more concerning environmental and ethical issues that must be addressed properly to ensure safe and acceptable use of GMOs³⁹.

Impact on Biodiversity and Ecosystem

The most important issue regarding the environmental concerns for genetically engineered wheat is its potential for an effect on biodiversity. Widespread use of GM wheat varieties could result in a loss of genetic diversity among the cultivated varieties and their wild relatives. The loss of diversity may make the crop more vulnerable to diseases and pests in the long term. Apart from this, gene flow from GM wheat to non-GM wheat or wild relatives would introduce genes in the population and, therefore, be ecologically unpredictable as engineered traits will disrupt local ecosystems.

Pesticide and Herbicide Resistance

Although genetically engineered varieties of wheat that resist pest and herbicides may minimize chemical usage, the risk of developing resistance in pest populations is very high. Single modes of action may lead to the development of resistant pest species and might be associated with an increase in pesticide consumption as well as its environmental consequences. Herbicide-resistant wheat also may lead to the change in weed populations, favoring more aggressive or invasive species.⁴⁰

Soil Health and Fertility

Long-term impacts of genetically modified wheat on soil health and fertility are also continually researched. Farming practices with GM crops often involve monoculture and significantly high input chemical fertilizers, reducing soil quality with the passage of time. Therefore, such genetic engineering of crops should be accompanied by sustainable agriculture practices that involve crop rotation, cover cropping, and organic amendments for long-term health in the ecosystems of the soils.

Consumer Health and Safety

The major safety concern of GM wheat is food intake by humans. Although GM wheat is tested for its safety and allergenicity, people continue to be concerned about health risks associated with the intake of GM foods. Open labeling and appropriate risk assessment will thus be important to address customer fear and establish confidence in such genetically modified products.

Ethical Considerations

The genetics of wheat raise the ultimate questions of ethics and morality. An example of this ethical debate has to do with the ownership and patenting of genetically modified organisms. Other issues regarding the seed patents of these giant agribusiness firms might impede the peasants’ right to seed saving and exchange, further perpetuating issues of food sovereignty and economic dependency. Questions about equitable access of genetic technology among small-scale farmers and developing countries arise. While the ability to produce crops with modified nutritional composition opens up ethical issues about the right to food and whether the developments would serve the interest of scientists or corporates to effectively deal with hunger and malnutrition globally, ensuring that such biotechnology produces respond to the needs of the poor population is a key to the satisfaction of ethical dilemmas.

Public Perception and Acceptance

Public opinion on genetically modified wheat is mixed and culturally, socially, and politically motivated. Consumers have misconceptions and little knowledge of genetic engineering, which contributes to the negative perception of GM products. Open public dialogue and communication between the public and scientists about the benefits and risks of GM wheat would help in acceptance and informed choice.

Regulation

Noting the importance of genetically engineered wheat, control over such comes as a crucial factor of environmental safety and public health. An effective regulatory framework, therefore, would entail the establishment and implementation of well-studied guidelines that take into account the potential environmental impact and ecological risks of GM crops, as well as food safety concerns. This calls for joint efforts on the part of governments and research institutions as well as active participation from stakeholders to obtain a regulatory environment that is balanced and conducive enough to encourage innovation while ensuring that protection is given to the environment and public interest.

Challenges and Limitations

Although the opportunity brought by genetic engineering to enhance the yield and resistance of wheat (Triticum aestivum) offers great advantages, various challenges and limitations have to be met in order for this to become a reality. These may pose a negative impact on the growth, adoption, and sustainability of GM wheat varieties in the future.

Regulatory Hurdles

The regulatory and approval frameworks around GMOs differ relatively vastly among the various countries, thus complicating the approval and commercialization process for genetically engineered wheat. Harsh regulations and long waiting times can increase the number of years that may elapse before new varieties are released to the marketplace and, consequently, slow response to agro challenges. Small biotech firms and individual researchers may find it particularly trying to cross the different regulatory frameworks.

Public Perception and Acceptance

Several challenges prevent the adoption of genetically modified wheat, including public concerns over its safety and environmental impact. Consumers, farmers, and lawmakers may take resistance in adopting GM wheat due to misinformation, fear of the unknown, and cultural attitudes toward biotechnology. Overcoming these perceptions requires effective communication and education for the facilitation of trust in the issues of the safety and benefits derived from GM wheat.

Dependence on Chemical Input

Though genetically engineered wheat might reduce the usage of some pesticides and herbicides, it could potentially increase reliance on others, such as fertilizers. Greater application for desirable attributes like high yield would raise environmental problems, including soil erosion and water pollution, if more fertilizers are used. Therefore, sustainable agriculture should be part of genetic engineering to minimize reliance on chemical inputs.

Technical Barriers of Development

Developing genetically engineered wheat is technically challenging and requires enormous investment. Scientists have to look for appropriate genes that should be targeted. They have to ensure that gene engineering is accurate so that expressed new traits are well stabilized and inherited in the new host plant. Furthermore, the introduced traits can also vary in their expressions due to many environmental factors when expressed as phenotypes. This has a tendency to make assessment of genetically modified varieties even more challenging to work with while in the field. This calls for rigorous research and tremendous investment in biotechnology.

Environmental Issues

Potential impacts on biodiversity and ecosystem dynamics are being researched, but already, future research and debate are steered toward the possibility of gene flow into wild relatives and the expected development of resistance in pest populations. Prudence and careful management and monitoring must be applied. Equally, the potential environmental benefits stemming from reduced use of pesticides and herbicides have to be weighed against the risks of reliance on single-gene traits.⁴¹

Economic Considerations

The economic viability of adopting GM varieties of wheat varies among different farm systems and locations. For instance, whereas the GM varieties can ensure more increased yields and lower input costs, the initial investments associated with biotechnology, seed purchase, and other regulations may act as a barrier for entry to many farming levels. Therefore, an economic analysis forms a prerequisite in determining the long-run profitability or sustainability of adopting genetically modified wheat, particularly among smallholder farmers in developing countries.

Future Directions   

The field of wheat genetic engineering continues to rapidly evolve – advances in biotechnology, computing tools, and an ever-deeper understanding of plant genetics are some of the drivers that could indicate promising future directions in amplifying the potential of genetically engineered wheat under increasingly pressing global challenges related to food security, climate change, and sustainable agricultural practices.

Integration of Multi-Omics Approaches

The incoming years will most likely bring much more employment of multi-omics approaches in integrated genomics, transcriptomics, proteomics, and metabolomics in research. Such holistic analyses will, step by step, further clarify gene-protein-metabolite interactions, thus opening the possibility for more precise engineering of wheat traits. Those insights are specifically to be applied in the development of varieties of wheat with improved stress tolerance, enhanced quality in terms of nutrition, and higher yield potential.⁴²

Advanced Gene Editing Technologies

Advanced gene editing technologies, such as CRISPR-Cas9, CRISPR-Cas12, and other next-generation genome editing tools, are transformative in wheat genetic engineering. Techniques that can make precise modification of specific genes make the techniques more efficient and limit the effects to what is desirable. Enhancements in these methods will likely be targeted at improving traits associated with disease resistance, drought tolerance, or nutritional content.⁴³

Climate Resilience Breeding

Once the forces of climate change are intensified, developing resistant wheat varieties for better environmental tolerance will be extremely important. Future research should be oriented to develop traits that can tolerate extreme temperatures, droughts, and salinity. This might include finding genes from wild relatives or other plant species that appear to be better at tolerating harsh conditions.⁴⁴

Nutritional Enhancement Focus

Biofortification of staples towards improving global malnutrition is going to remain an important focus area. Next generation biotech applications will be directed at nutritional content improvement in wheat through increased essential vitamins, minerals, and amino acids. Interdisciplinary collaboration with nutrition scientists will also be significant with respect to ensuring that wheat varieties modified become nutritionally adequate for vulnerable populations.⁴⁵

Sustainable agricultural practices

As demand for sustainability in genetic engineering of wheat grows, the focus of research will also shift towards development of low-input chemical varieties such as reduced fertilizer and pesticide use without sacrificing or losing yield. More sustainable wheat production systems would be achieved through techniques such as integrating genetic engineering with agroecological practices, cover cropping, and organic farming methods.

Increased Collaboration and Public Engagement

Collaboration between researchers, industry stakeholders, policymakers, and the public will be significant steps forward for progress in the genetic engineering of wheat. Public engagement into these debates on the pros and cons of genetically engineered crops will cultivate trust and acceptance. Collective work should also target improvements in regulatory matters as well as involve equal access to biotechnological innovations for smallholder farmers.

Synthetic Biology Application

Synthetic biology, for instance, may be used in the engineering of wheat. This promises new traits and changes the existing ones. These new biological parts and systems can be prepared and assembled to create varieties of wheat that will possess advanced features such as enhanced biotic stress resistance, abiotic stress, and greater uptake of nutrients.  

Precision Agriculture and Data-Driven Approaches

The integration of precision agricultural technologies with genetic engineering will result in resource use efficiency and better management of wheat crops. Data analytics, machine learning, and remote sensing will help farmers make informed decisions in real time regarding conditions on soil health, weather patterns, and performance of the crops. Genetic engineering can complement this by giving farmers wheat varieties tailored to their local conditions.

Regenerative Agriculture Practices

Future areas include genetic engineering in union with the application of principles of regenerative agriculture. This is agriculture for the re-restoration of soils to health with biodiversity. Producing wheat varieties adapted for regenerative systems will enhance sustainable food production. Soil cultivation, building carbon sequestration through soils, and enhancing biodiversity in wheat farming will be the focus of the research.⁴⁶

Global Cooperation for Food Security

International cooperation and knowledge sharing are required to tackle worldwide issues in food security. Future work should relate to the development of networks among researchers, governments, and NGOs with the aim of best practices, technological diffusion, and research finding. Results of combined programs shall improve wheat varieties for different regions and ensure technological progress in genetic engineering to reach more farmers around the world.⁴⁷ 

Conclusion   

Genetic engineering is a revolutionary approach to crop yield improvement, especially given the universal challenges of climate change, population growth, and ensuring food security. New crop designs, using the technically recently booming technologies of CRISPR, transgenic methods, and RNA interference, have been found and are on their way towards better more productive, sustainable, and hardier crops. However, there is a lot more of environmental, ethical, and regulatory work that has to be done responsibly and equitably with respect to genetic engineering in agriculture. Continuing research will mean to stir innovation, allowing genetic engineering to revolutionize agriculture, for a better food-secure future. 

Acknowledgement

The authors would like to thank Dr. D. Y. Patil Institute of Technology for necessary support. 

Funding Sources

The author(s) received no financial support for the research, authorship, and/or publication of this article. 

Conflict of Interest

The authors do not have any conflict of interest. 

Data Availability Statement

This statement does not apply to this article. 

Ethics Statement

This research did not involve human participants, animal subjects, or any material that requires ethical approval. 

Author Contributions

Lalit Narendra Patil : Conceptualization, Writing – Original Draft

Santosh D. Sancheti : Data Collections

Atul Ashok Patil : Critical Review and Supervision

Sarika Atul Patil : Supervision and Final Approval

Vikash K. Agrawal: Writing – Original Draft

References

1. Das D, Singha DL, Paswan RR, et al. Recent advancements in CRISPR/Cas technology for accelerated crop improvement. Planta. 2022;255(5):109. doi:10.1007/s00425-022-03894-3
   CrossRef
2. Rahman SU, McCoy E, Raza G, Ali Z, Mansoor S, Amin I. Improvement of Soybean; A Way Forward Transition from Genetic Engineering to New Plant Breeding Technologies. Mol Biotechnol. 2023;65(2):162-180. doi:10.1007/s12033-022-00456-6
   CrossRef
3. Achmad T. Nugraha, Gunawan Prayitno, Abdul W. Hasyim, Fauzan Roziqin. Social Capital, Collective Action, and the Development of Agritourism for Sustainable Agriculture in Rural Indonesia. Evergreen. 2021;8(1):1-12. doi:10.5109/4372255
   CrossRef
4. Mei Y, Wang Y, Chen H, Sun ZS, Ju XD. Recent progress in CRISPR/Cas9 technology. Journal of Genetics and Genomics. 2016;43(2):63-75.
   CrossRef
5. Singh V, Braddick D, Dhar PK. Exploring the potential of genome editing CRISPR-Cas9 technology. Gene. 2017;599:1-18.
   CrossRef
6. Hartenian E, Doench JG. Genetic screens and functional genomics using CRISPR /Cas9 technology. The FEBS Journal. 2015;282(8):1383-1393. doi:10.1111/febs.13248
   CrossRef
7. Rao MJ, Wang L. CRISPR/Cas9 technology for improving agronomic traits and future prospective in agriculture. Planta. 2021;254(4):68. doi:10.1007/s00425-021-03716-y
   CrossRef
8. Prosekov AY, Ivanova SA. Food security: The challenge of the present. Geoforum. 2018;91:73-77.
   CrossRef
9. Eş I, Gavahian M, Marti-Quijal FJ, et al. The application of the CRISPR-Cas9 genome editing machinery in food and agricultural science: Current status, future perspectives, and associated challenges. Biotechnology advances. 2019;37(3):410-421.
   CrossRef
10. Zhan T, Rindtorff N, Betge J, Ebert MP, Boutros M. CRISPR/Cas9 for cancer research and therapy. In: Seminars in Cancer Biology. Vol 55. Elsevier; 2019:106-119. Accessed September 28, 2024. https://www.sciencedirect.com/science/article/pii/S1044579X17302742
    CrossRef
11. Ruiz-Real JL, Uribe-Toril J, Torres Arriaza JA, de Pablo Valenciano J. A look at the past, present and future research trends of artificial intelligence in agriculture. Agronomy. 2020;10(11):1839.
    CrossRef
12. Dale PJ, Clarke B, Fontes EM. Potential for the environmental impact of transgenic crops. Nature biotechnology. 2002;20(6):567-574.
    CrossRef
13. Motes WC. Modern agriculture and its benefits–Trends, implications and outlook. Global Harvest Initiative, Washington, DC. Published online 2010. Accessed September 28, 2024.
    https://putramooc.upm.edu.my/pluginfile.php/526/mod_resource/content/1/Attachment%201.pdf
14. Tang G, Qin J, Dolnikowski GG, Russell RM, Grusak MA. Golden Rice is an effective source of vitamin A. The American journal of clinical nutrition. 2009;89(6):1776-1783.
    CrossRef
15. Oliver MJ. Why we need GMO crops in agriculture. Missouri medicine. 2014;111(6):492.
16. Wilson RC, Doudna JA. Molecular Mechanisms of RNA Interference. Annu Rev Biophys. 2013;42(1):217-239. doi:10.1146/annurev-biophys-083012-130404
    CrossRef
17. Taliansky M, Samarskaya V, Zavriev SK, Fesenko I, Kalinina NO, Love AJ. RNA-based technologies for engineering plant virus resistance. Plants. 2021;10(1):82.
    CrossRef
18. Kawasaki H, Taira K, Morris KV. siRNA Induced Transcriptional Gene Silencing in Mammalian Cells. Cell Cycle. 2005;4(3):442-448. doi:10.4161/cc.4.3.1520
    CrossRef
19. Kim DS, Zhang J. Strategies to improve the efficiency of RNAi-mediated crop protection for pest control. Entomol Gen. Published online 2022. Accessed October 3, 2024. https://www.researchgate.net/profile/Dae-Sung-Kim/publication/363103997 _Strategies_to_improve _the_efficiency_of_RNAi-mediated_crop_protection _for_pest_control/links/ 63c3a18fe922c50e 9997867e/ Strategies-to-improve-the-efficiency-of-RNAi-mediated-crop-protection-for-pest-control.pdf
20. Ai G, Liu J, Fu X, et al. Making Use of Plant uORFs to Control Transgene Translation in Response to Pathogen Attack. BioDesign Res. 2022;2022:9820540. doi:10.34133/2022/9820540
    CrossRef
21. Kim DH, Rossi JJ. RNAi Mechanisms and Applications. BioTechniques. 2008;44(5):613-616. doi:10.2144/000112792
    CrossRef
22. Paoletti MG, Pimentel D. Genetic engineering in agriculture and the environment. BioScience. 1996;46(9):665-673.
    CrossRef
23. Bhullar NK, Gruissem W. Nutritional enhancement of rice for human health: the contribution of biotechnology. Biotechnology advances. 2013;31(1):50-57.
    CrossRef
24. Hawkins NJ, Bass C, Dixon A, Neve P. The evolutionary origins of pesticide resistance. Biological Reviews. 2019;94(1):135-155. doi:10.1111/brv.12440
    CrossRef
25. Shook GE. Selection for disease resistance. Journal of Dairy Science. 1989;72(5):1349-1362.
    CrossRef
26. Luo W, Song F, Xie Y. Trade-off between tolerance to drought and tolerance to flooding in three wetland plants. Wetlands. 2008;28(3):866-873. doi:10.1672/07-225.1
    CrossRef
27. Gressel J. Herbicide tolerance and resistance: alteration of site of activity. In: Weed Physiology. CRC press; 2018:159-189. Accessed October 19, 2024.https://www.taylorfrancis.com/chapters/edit/ 10.1201/9781351077736-7/herbicide-tolerance-resistance-alteration-site-activity-gressel
    CrossRef
28. Garcia A, Gaju O, Bowerman AF, et al. Enhancing crop yields through improvements in the efficiency of photosynthesis and respiration. New Phytologist. 2023;237(1):60-77. doi:10.1111/nph.18545
    CrossRef
29. Türkan I, Demiral T. Recent developments in understanding salinity tolerance. Environmental and Experimental Botany. 2009;67(1):2-9.
    CrossRef
30. YUAN L ping. Hybrid rice achievements, development and prospect in China. Journal of Integrative Agriculture. 2015;14(2):197-205.
    CrossRef
31. Ranum P, Peña‐Rosas JP, Garcia‐Casal MN. Global maize production, utilization, and consumption. Annals of the New York Academy of Sciences. 2014;1312(1):105-112. doi:10.1111/nyas.12396
    CrossRef
32. Enayati AA, Ranson H, Hemingway J. Insect glutathione transferases and insecticide resistance. Insect Molecular Biology. 2005;14(1):3-8. doi:10.1111/j.1365-2583.2004.00529.x
    CrossRef
33. Bonny S. Genetically Modified Herbicide-Tolerant Crops, Weeds, and Herbicides: Overview and Impact. Environmental Management. 2016;57(1):31-48. doi:10.1007/s00267-015-0589-7
    CrossRef
34. Nezhadahmadi A, Prodhan ZH, Faruq G. Drought Tolerance in Wheat. Boyd RS, Rajakaruna N, eds. The Scientific World Journal. 2013;2013(1):610721. doi:10.1155/2013/610721
    CrossRef
35. Peralta‐Yahya PP, Keasling JD. Advanced biofuel production in microbes. Biotechnology Journal. 2010;5(2):147-162. doi:10.1002/biot.200900220
    CrossRef
36. Kimura MT. Cold and heat tolerance of drosophilid flies with reference to their latitudinal distributions. Oecologia. 2004;140(3):442-449. doi:10.1007/s00442-004-1605-4
    CrossRef
37. Fageria NK, Baligar VC. Enhancing nitrogen use efficiency in crop plants. Advances in agronomy. 2005;88:97-185.
    CrossRef
38. Anfinrud MN. Planting Hybrid Seed Production and Seed Quality Evaluation. In: Schneiter AA, ed. Agronomy Monographs. American Society of Agronomy, Crop Science Society of America, Soil Science Society of America; 2015:697-708. doi:10.2134/agronmonogr35.c14
    CrossRef
39. Gupta A. Ethical and policy concerns pertaining to rice landraces in Asia. Bangladesh Journal of Bioethics. 2011;2(1):7-12.
    CrossRef
40. Swift MJ. Human impacts on biodiversity and ecosystem services: an overview. MYCOLOGY SERIES. 2005;23:627.
    CrossRef
41. Lalit N. Patil, Hrishikesh P. Khairnar, J. A. Hole, et al. An Experimental Investigation of Wear Particles Emission and Noise Level from Smart Braking System. Evergreen. 2022;9(3):711-720. doi:10.5109/4843103
    CrossRef
42. Shahrajabian MH, Sun W. Survey on Multi-omics, and Multi-omics Data Analysis, Integration andApplication. CPA. 2023;19(4):267-281. doi:10.2174/1573412919666230406100948
    CrossRef
43. Gantz VM, Akbari OS. Gene editing technologies and applications for insects. Current opinion in insect science. 2018;28:66-72.
    CrossRef
44. Langridge P, Braun H, Hulke B, Ober E, Prasanna BM. Breeding crops for climate resilience. Theor Appl Genet. 2021;134(6):1607-1611. doi:10.1007/s00122-021-03854-7
    CrossRef
45. Lindsay DG. The nutritional enhancement of plant foods in Europe ‘NEODIET.’ Trends in Food Science & Technology. 2000;11(4-5):145-151.
    CrossRef
46. Lalit N. Patil, Hrishikesh P. Khairnar. Investigation of Human Safety Based on Pedestrian Perceptions Associated to Silent Nature of Electric Vehicle. Evergreen. 2021;8(2):280-289. doi:10.5109/4480704
    CrossRef
47. Kornher L, Kalkuhl M. The gains of coordination-When does regional cooperation for food security make sense? Global Food Security. 2019;22:37-45.
    CrossRef