Fungal Antagonism and Plant Growth Promoting Efficacy of Aspergillus oryzaeAVNF4 isolated from the Rhizome of Curcuma longa on Lycopersicum esculantum L. (tomato)

Narmada Sanneboyinaand Amrutha Valli Audipudi^*

Department of Microbiology, Acharya Nagarjuna University, Guntur;, Andhra Pradesh, India.

Corresponding Author E-mail:audipudiamrita@gmail.com

DOI : http://dx.doi.org/10.12944/CARJ.12.3.11

Abstract:

The present study aimed to develop liquid bioformulation of Aspergillus oryzae AVNF4, a Curcuma longa rhizome endophytic fungus with antagonistic activity against Fusarium oxysporum causing tomato wilt and plant growth promotion of Lycopersicum esculantum. Fungal antagonism, Indole 3- acetic acid production, ammonia and inorganic phosphate solubilisation and GC-MS analysis of antifungal metabolites of A. oryzae AVNF4 were analysed. Liquid bio formulation of A. oryzae AVNF4 (LBF) with enhanced production of IAA, ammonia and inorganic phosphate solubilisation has developed by optimising the culture medium. Seed bacterisation of tomato seeds with LBF for 48h followed by foliar application from the second week onwards showed a significant increase in germination percentage (Gp) and germination index (GI) along with biomass, plant height, shoot length, root length, root/shoot ratio, number of leaves, and soluble protein and reduced sugars in LBF treated plants compared to untreated control. Presence of 1,3-Dioxolane, 2-(1-propenyl)-, L-Prolinamide, 5-oxo-L-prolyl-L-phenylalanyl-4-hydroxy-, Pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro-, dl-Mevalonic acid lactone, Hydro cinnamic acid, Oleic acid, 9-Octadecenoic acid (Z)-, Pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro-3-(phenylmethyl)-, 1-Allylazetidine, 4H-Pyran-4-one, 2,3-dihydro-3,5-dihydroxy-6-methyl-, 5-Pyrrolidino-2-pyrrolidone, Pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro-3-(2-methyl propyl)-, 9-Octadecenoic acid (Z)-, hexyl ester, Dehydromevalonic lactone, Acetaldehyde, (3,3-dimethyl cyclohexylidene)-, (Z)- in ethyl acetate extract of AVNF4 reported to be the pivotal compounds responsible for fungal antibiosis and to promote growth in tomato seedlings. The field performance of LBF of AVNF4 indicated that A. oryzae AVNF4 is a promising plant growth-promoting endophyte with fungal antagonism against Fusarium wilt.

Keywords:

Aspergillus oryzae; Endophyte; Fungal antagonism; Fusarium wilt; GC-Ms; Liquid bioformulation; Lycopersicon esculentum; Seedling vigour.

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