Molecular Characterization and Plant Growth Promotion Potential of Paenibacillus Dendritiformis Endophyte Isolated from Tecomella Undulata (Roheda)

Nisha Choudhary, Mahima Choudhary, Sunidhi Mishra and Rakesh Kumar Verma*

Department of Biosciences, School of Liberal Arts and Sciences, Mody University of Science and Technology, Lakshmangarh, Sikar, Rajasthan, India.

Corresponding Author Email:rkwat4@yahoo.com

Abstract:

In this study, we have isolated a bacterial endophyte Paenibacillus dendritiformis strain RAE13 (Accession number: OR259131) from the leaves of Tecomella undulata (Roheda) plant. The identification of bacterial species was carried out using 16s-rDNA ribotyping. Subsequently, the isolated bacterial strain was gauged for its potential to endorse plant growth through various mechanisms such as nitrogen fixation, IAA production, HCN synthesis, siderophore generation, and ammonia production. Furthermore, the evaluation focused on the endophyte's capacity for producing extracellular enzymes, including cellulase, chitinase, protease, amylase, and catalase. The endophyte exhibited to synthesize an average of 18±0.375 μg/ml of indole-3-acetic acid (IAA) after being subjected to a concentration of 5 mg/ml of tryptophan over a 14-day incubation period. The endophytic isolate RAE 13 produced an average of 42.4±0.004 μg/ml of Gibberellin, solubilized phosphate in the range of 70.2 μg/ml to 135.5 μg/ml, and produced an average of 45.5 μg/ml of ammonia. The phylogenetic analysis unveiled that the isolated strain RAE13 had a common ancestor and had a maximum nucleotide sequence similarity of 98.30% with Paenibacillus sp isolates of Uttar Pradesh, India. To diminish the consumption of chemicals in conventional farming, the results indicated that the isolated endophyte had great potential as a plant growth-stimulating inoculant. Henceforward, utilization of these extracellular enzymes for medical and industrial applications will be highly beneficial. Additionally, it could enhance plant tolerance to challenging environmental circumstances including drought and high temperatures.

Keywords:

Endophytes; Extracellular Enzymes; Indole Acetic Acid; Plant Growth Promotion; Phylogeny

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