Screening and Optimization of IAA Production by PGPR isolated from Rhizosphere of a Pterocarpus marsupium Roxb. and their Effect on Plant Growth

Vaishali Sanjay Randive^1,2, Snehal Nitin Agnihotri² and Rani Babanrao Bhagat^3*

¹Department of Biotechnology, Modern College of Arts, Science and Commerce College, Ganeshkhind, Pune Maharashtra India.

²Department of Microbiology, Tuljaram Chaturchand College, Baramati, Dist- Pune. Maharashtra, India

³Department of Botany, Baburaoji Gholap College of Arts, Commerce, and Science, Sangvi, Pune, Maharashtra, India.

Corresponding Author Email:rb_botany@rediffmail.com

DOI : http://dx.doi.org/10.12944/CARJ.12.1.26

Abstract:

Indole Acetic Acid (IAA) production is important attribute of PGPR that promote plant growth and development. The rhizosphere is hotspot in the soil that harbors PGPR. The present study was aimed with isolation and screening of IAA producing bacteria from the rhizosphere of Pterocarpus marsupium Roxb. Optimum culture conditions (pH, temperature, incubation period and L-tryptophan concentration for IAA production were studied for selected isolates and their effect on wheat growth and root development  was  evaluated. Among twenty four IAA producing isolates five isolates (Et1, Rp1, Rp5, Rp6, and Rp9) produced maximum IAA in range of 50-70 μg/mL and was used in optimization studies. Maximum IAA was produced in 96 hours of incubation, at pH 7 and with 0.1mg/mL of L-tryptophan by all five isolates. 30^oC is the most suitable temperature for Et1, Rp1, Rp5, Rp9; whereas Rp6  produced   nearly same amount of  IAA at wide range of  temperature 30-35^oC (77-84.12 μg/mL) and  at pH 7-8 ( 73-74μg/mL). Out of the five isolates, Rp6 exhibits the highest potential, having a maximum IAA of 84.12 μg/mL at 35°C and pH 7.  Although tryptophan influences IAA synthesis but at higher concentration of tryptophan inhibits IAA synthesis. To validate the production of IAA, crude extracts were analyzed using thin layer chromatography (TLC). A spot of standard IAA with the same R_f value (0.91) was found to match a specific spot from the crude IAA.

Keywords:

IAA; PGPR; Pterocarpus marsupium; Rhizosphere; Tryptophan

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