Antibacterial activity and Phytochemical screening of Tagetus erecta L. and Pelargonium graveolens against Xanthomonas axonopodis pv. punicae

G. L. Aruna*

Department of Microbiology, Maharani’s Science College for Women, Mysore, Karnataka, India

Corresponding Author E-mail: microarunagl@gmail.com

DOI : http://dx.doi.org/10.12944/CARJ.11.2.24

Abstract:

The antibacterial activity of selected medicinal plants namely Tagetus erecta L. and Pelargonium graveolens extracts was studied against Xanthomonas axonopodis pv. punicae, the causative agent of bacterial blight of Punica granatum. The plants are selected based on their traditional use as medicinal plants.The water, ethanol and methanol extracts of Tagetusr erecta L. and Pelargonium graveolens were used for antibacterial activity assay by Kirby Bauer disc diffusion method. Among these extracts, only methanol extract of Taget erecta L.has showed substantial antibacterial activity against Xanthomonas axonopodis pv. punicae and all other extracts showed less antibacterial activity. The phytochemical constituents such asalkaloids, flavonoids, tannins, proteins, resins, proteins, saponins, terpenoids, steroids, and glycosides were found to be present in selected plant extracts as per preliminary phytochemical investigation. Only methanol extract showed maximum inhibition zone and all other extracts of Tagetus erecta and extracts of Pelargonium graveolens have showed less inhibition activity against Xanthomonas axonopodis pv. Punicae. The Minimum inhibitory concentration and Minimum bacteriocidal concentration of Tagetus erecta methanol extract was found to be 25 mg/ml and 50 mg/ml respectively. Our study revealed the possible application of Tagetus erecta L. methanol extract in the management of Bacterial blight of Punica granatum.           

Keywords:

Anti bacterial activity; Minimum bacteriocidal concentration; Phytochemical constituents; Tannins; Tagetus erecta L; Xanthomonas axonopodis

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