Oryzalin and Gamma Radiation Induced Polyploidization in Garden Balsam Plants (Impatiens balsamina L.) In Vitro

Made Ria Defiani*, Ida Ayu Astarini, Eniek Kriswiyanti

Department of Biology, Faculty of Mathematics and Natural Sciences, Udayana University, Bali, Indonesia

Corresponding author email: maderia@unud.ac.id

DOI : http://dx.doi.org/10.12944/CARJ.5.1.01

Abstract:

The aim of the research was to create genetic variation on garden balsam using oryzalin and Gamma radiation.  Mutagenic agent may improve plant variation.  Garden balsam seeds were treated by oryzalin (0%, 0.01%, 0.02%) then the seedlings were planted in the field four times to gain seeds  generation M4. Seedlings of M4 (7 days of age) were radiated by gamma ray ⁶⁰Co (0, 5, 10,  15 Gy) and planted in the field to collect seeds for in vitro study to induce a new type of mutant plant.    In vitro methods was conducted to achieved rapid micropropagation of Impatiens balsamina L. mutant plants. Growth percentage of seedlings reached 90% by gibberellin 0.01 ppm.  Leaf section used for explant and cultured in MS media enriched by 0.5 ppm NAA and 0.5 ppm BAP aseptically. Shoots were regenerated on 6 weeks after cultured however some shoots become vitrification. Number of chromosome varied (mixoploid) on treated plants (M5).  Form of secondary metabolites ( alkaloids and terpenoids ) in the roots extract was not changed by oryzalin and gamma radiation. Mixoploid explants showed variation in morphology and some treatments only had little shoots and a treatment has vigorous roots.  Control plants have shoot and callus.

Keywords:

Gamma radiation; In vitro; In vivo; Mixoploid; Oryzalin; Vitrification

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