DNA Profiling of the Threatened Himalayan Herb Polygonatum verticillatum L. Using Cross-transferred Betula SSR Markers

Pitamber Dutt Sharma¹ and Vikas Sharma^2*

¹Department of Life Sciences, Sant Baba Bhag Singh University Khiala, Jalandhar, Punjab, India.

²Department of Agriculture, Sant Baba Bhag Singh University Khiala, Jalandhar, Punjab India.

Corresponding Author E-mail:vikasam@gmail.com

DOI : http://dx.doi.org/10.12944/CARJ.12.2.36

Abstract:

Polygonatum verticillatum is an important Himalayan herb that is used in different medicine systems for improving health and curing many diseases. Herein, simple sequence repeat (SSR) marker characterization of this plant species was performed using cross-transferred SSR markers of a distantly related species Betula utilis. Among the 25 SSR markers tested, 13 generated clearly distinguishable alleles. Of these, 12 SSR primers were polymorphic and 1 was monomorphic. All the 12 markers collectively amplified 42 alleles. The average value of 3.5 alleles was observed. The size of alleles ranged from 100 - 600 bp. The mean polymorphism information content (PIC) was 0.459, and mean marker index was 1.61. The dendrogram clustered all the studied accessions into three groups according to geographical locations. The results showed high genetic diversity in the populations of P. verticillatum in Indian Himalayan region. SSR marker exhibited good amplification in distantly related species. The SSR markers used in the present work can help diversity and breeding research of P. verticillatum in coming days. The results of present work will be helpful for characterization, conservation, management and improvement of the germplasm of this plant in the future.

Keywords:

Betula utilis; Cross-transferability; Genetic Diversity; Polygonatum verticillatum; Simple Sequence Repeat (SSR)

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