Molecular Identification, Antagonistic Assay and Enzyme Profiling of Selected Trichoderma Isolates

Utkarsh Singh Rathore^{1, 2*}, Rudra Pratap Singh¹, Sonika Pandey² and Raj Kumar Mishra2

¹Division of Plant Pathology, Bhagwant University, Ajmer, Rajasthan, India.

²Division of Crop Protection, ICAR-Indian Institute of Pulses Research, Kanpur, India.

Corresponding Author E-mail: singh.utkarsh1499@gmail.com

DOI : http://dx.doi.org/10.12944/CARJ.12.2.20

Abstract:

The aim of this study was to find and characterize Trichoderma isolates by antagonistic and enzymatic activity to evaluate their potential as biocontrol agents against Dry root rot (DRR). Trichoderma isolates were isolated from thepulses rhizosphere of different districts of Uttar Pradesh. Twenty one Trichoderma isolates were identified using ribosomal DNA internal transcribed spacer (ITS) regions and translation elongation factor 1-alpha (tef1). In addition, enzymatic profiling of Trichoderma isolates was done indicated strong cell wall degrading enzyme activities and plant growth promoting traits of Trichoderma isolates. Overall, our results suggested that the isolated Trichoderma spp. have prodigious potential for plant growth promotion and can be used as biocontrol agents against dry root rot.

Keywords:

Biocontrol; Chickpea; Growth promotion; Pulses; Trichoderma

Download this article as: 

[ HTML Full Text]