Effects of Plant Growth Promoting Halotolerant Pseudomonas Aeruginosa JCM 5962 with Hydrocarbon Degradation Ability, Isolated from Sundarbans Mangrove Area in West Bengal, on Abelmoschus esculentus (Okra) Plant Growth

Biswajit Saha¹ and Aparna Sen^2*

¹Department of Microbiology, Bijoy Krishna Girls’ College, Howrah, West Bengal, India

²Department of Microbiology, Lady Brabourne College, Kolkata, West Bengal, India

Corresponding Author E-mail: aparnasenlbc@gmail.com

Abstract:

Plant growth promoting rhizobacteria (PGPR) play a key role in sustainable agricultural practices leading to increased crop productivity. Moreover, PGPR with ability to diminish abiotic stresses like salinity and hydrocarbon contamination in soil, can be developed into potent biofertilizers with maximum ecological benefits. Sundarbans mangrove region in West Bengal, a natural reservoir of diverse microbiota is an important source of PGPR adapted to high salinity and other abiotic stresses like hydrocarbon contamination due to oil spillage and water transport systems, rendering the soil unsuitable for farming. In the present study, a potent PGPR has been isolated from rhizospheric soil of Matla riverbed in mangrove areas of Sundarbans, with simultaneous nitrogen fixing, phosphate solubilizing and plant hormone like indole acetic acid (IAA) producing properties as well as high salt tolerance and hydrocarbon bioremediation abilities. The strain has been identified as Pseudomonas aeruginosa JCM 5962 (NCBI Accession number MK544832.1) on the basis of 16S rRNA analysis. The isolated Pseudomonas aeruginosa strain showed atmospheric nitrogen fixation (3612 ± 2 mg N/ Kg of soil), highest phosphate solubilization index of 3.0 ± 0.06 and 37.14 µg/mL of IAA production. This potent strain also showed salt tolerance upto 7% in culture broth and an uptake of 18.72% of salt. Highest hydrocarbon degradation was shown by this strain in presence of diesel as the sole carbon source. The isolated Pseudomonas aeruginosa strain showed overall improvement in growth of Abelmoschus esculentus (Okra) plants in pot experiments in different conditions like absence of any abiotic stress, presence of 5% salt stress and presence of 1% diesel contaminant. These results indicate that Pseudomonas aeruginosa JCM 5962 can be developed as a potent biofertilizer to be used in agricultural lands of Sundarbans mangrove regions and other areas which are plagued by high salinity and increasing hydrocarbons, particularly petroleum contamination.

Keywords:

Biofertilizer; Hydrocarbon degradation; Nitrogen-fixation; PGPR; Phosphate Solubilization; Salinity

[ HTML Full Text]