Advances in the Micropropagation and Genetic Transformation of Abelmoschus esculentus (L.) Moench for Insect Resistance

Melvin A Daniel, V. Duraipandiyan and S. Maria Packiam^*

Division of Plant Biotechnology, Entomology Research Institute, Loyola College, Chennai, India

Corresponding Author E-mail: eripub@loyolacollege.edu

DOI : http://dx.doi.org/10.12944/CARJ.10.3.08

Abstract:

Abelmoschus esculentus (L.) Moench, known as okra, is a common vegetable crop in many diets and serves as a nutrient-rich source. It has a high content of protein, vitamins, minerals and compounds of high medicinal value. India tops in the consumption of pods and ranks first among the worldwide total production. It is now widely cultivated in many countries. Among the factors that hamper okra's marketable fruit yield, insect pests are the major ones. As numerous pests attack vegetables, controlling insect pests is one of the key elements to improve the yield of this crop. A workable approach for improving okra yield is micropropagation. It has been employed for a variety of things, including as large multiplication, inducing somaclonal variation to improve the desirable agronomic traits, maintaining certain genotypes, and genetic modification utilising molecular techniques. In this review, we highlight the most significant research on the micropropagation of okra, which is mediated by a variety of regeneration responses. The media and growth regulators for each of the approaches discussed, we go through how transformation techniques for insect resistance have been made possible via micropropagation. Utilizing this technology might be a workable plan to add genes and enhance particular features. Studying molecular pathways is another option provided by genetic transformation. This offers benefits for developing breeding programmes and optimising field production especially the effective use of CRISPR in genetically diverse lepidopteran insects opened options to study gene functions, insect modification, and pest management.

Keywords:

Abelmoschus esculentus; Bacillus thuringiensis; Agrobacterium-mediated transformation; Insect resistance; plant tissue culture

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